CST, 30817S, GAP43 (E1U1D) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GAP43/Neuromodulin. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GAP43 (E1U1D) Mouse Monoclonal Antibody (CST #30817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 47235 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GAP43 (E1U1D) Mouse Monoclonal Antibody recognizes endogenous levels of total GAP43 protein. This antibody detects a non-specific band of unknown origin at 110 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse GAP43 protein. Background GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7). Alternate Names Axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; GAP43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; NEUM; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; pp46; protein F1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38, 43 Source/Isotype: Mouse IgG1 kappa