CST, 3083S, Phospho-CSF-1R/M-CSF-R (Tyr546) Antibody

Polyclonal Antibody for studying CSF1R (Tyr546) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CSF-1R/M-CSF-R (Tyr546) Antibody (CST #3083) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-CSF-1R/M-CSF-R (Tyr546) Antibody detects transfected levels of CSF-1R/M-CSF-R only when phosphorylated at Tyr546. This antibody may cross-react with other tyrosine-phosphorylated protein tyrosine kinases. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr546 of human CSF-1R/M-CSF-R. Antibodies are purified by protein A and peptide affinity chromatography. Background Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). Tyr546 is a major autophosphorylation site in the juxtamembrane domain of the M-CSF receptor. Phosphorylation of Tyr546 plays an important role in the activation of M-CSF (9) and may provide a binding site for downstream signaling components (10). Alternate Names BANDDOS; C-FMS; CD115; CD115 antigen; colony stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; CSF1R; CSFR; FIM2; FMS; FMS proto-oncogene; HDLS; M-CSF-R; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; Proto-oncogene c-Fms Specification REACTIVITY: H M SENSITIVITY: Transfected Only MW (kDa): 175 SOURCE: Rabbit