CST, 30853S, Emerin (D3B9G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Emerin. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Emerin (D3B9G) Rabbit Monoclonal Antibody (CST #30853) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Emerin (D3B9G) Rabbit Monoclonal Antibody recognizes endogenous levels of total emerin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human emerin protein. Background Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to β-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that emerin is required for HIV-1 infectivity (9). Mutations in the gene encoding emerin ( ) are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10). Alternate Names EDMD; EMD; Emerin; LEM domain containing 5; LEMD5; STA Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 30, 35 Source/Isotype: Rabbit IgG