CST, 3086T, PASK (C70B2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PASK mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PASK (C70B2) Rabbit Monoclonal Antibody (CST #3086) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PASK (C70B2) Rabbit Monoclonal Antibody detects endogenous levels of total PASK protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln295 of mouse PASK protein. Background PASK is a serine/threonine kinase that contains two PAS (Per-Arnt-Sim) domains (1). Its kinase activity is up-regulated by autophosphorylation of the activation loop within its catalytic domain and is inhibited by one of the PAS domains (1). Studies found that the yeast homolog of PASK phosphorylates and inhibits two enzymes required for glycogen biosynthesis: UDP-glucose pyrophosphorylase and glycogen synthase, resulting in the decrease of carbohydrate storage (2). Further studies showed that increased glucose levels activate PASK activity and enhance its expression in pancreatic β-cells (3). PASK is essential for the glucose-stimulated expression of preproinsulin and Pdx1, suggesting its role in the regulation of genes involved in pancreatic β-cell functions (3). PASK was shown to be critical for glucose-stimulated insulin secretion in pancreatic β-cells (4). The absence of PASK also protects animals from obesity and insulin resistance when they are fed a high-fat diet (4). These findings suggest that PASK functions as an important metabolic sensor in various cells (4). Alternate Names Kiaa0135; mKIAA0135; OTTMUSP00000024404; PAS domain containing serine/threonine kinase; PAS domain-containing serine/threonine-protein kinase; PAS-kinase; Pask; PASKIN Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG