CST, 30871S, CRTAM (F5I5L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRTAM. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CRTAM (F5I5L) Rabbit Monoclonal Antibody (CST #30871) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CRTAM (F5I5L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRTAM protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CRTAM protein. Background Class I-restricted T cell-associated molecule (CRTAM) is a type I transmembrane glycoprotein that plays a role in T cell development, proliferation, and adhesion (1). CRTAM is transiently expressed on the surface of activated CD8 T cells and natural killer (NK) cells, where it binds to its ligand CADM1 (2,3). CRTAM is also expressed on particular subsets of activated CD4 T cells and is required for the differentiation of CD4 cytotoxic T cells (CTLs) (4-6). CRTAM binding to CADM1 has been shown to enhance the adhesive capacity and cytotoxicity of CD8 T cells and NK cells (2). It also promotes retention and proliferation of CTLs in draining lymph nodes via interaction with CADM1-expressing dendritic cells (DCs) (7). Interestingly, elevated CRTAM expression has been found to be associated with increased survival in patients with triple negative breast cancer (TNBC), suggesting its use as a potential biomarker for immunotherapy efficacy (8). Alternate Names CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; Class-I MHC-restricted T-cell-associated molecule; CRTAM; cytotoxic and regulatory T cell molecule; Cytotoxic and regulatory T-cell molecule; O95727 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG