CST, 30894T, ZFP36L1 (E6L6S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZFP36L1/BRF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ZFP36L1 (E6L6S) Rabbit Monoclonal Antibody (CST #30894) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ZFP36L1 (E6L6S) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZFP36L1 protein. This antibody does not cross-react with ZFP36L2 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro281 of human ZFP36L1 protein. Background ZFP36L1, also known as butyrate response factor-1 (BRF1), and ZFP36L2, also known as butyrate response factor-2 (BRF2), both belong to the TIS11 family of CCCH zinc-finger proteins (1). This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (AREs) found in the 3'-untranslated regions of mRNAs and promote deadenylation and rapid degradation by the exosome (2,3). These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors, and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3, and IL-6 (4,5). Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation (6-8). Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of ZFP36L1 at Ser92, which results in binding by 14-3-3 protein and inactivation of ZFP36L1 (9). ZFP36L1 and L2 have also been shown to promote cell quiescence in developing B lymphocytes, promoting VDJ recombination (10). Alternate Names BERG36; BRF1; Butyrate response factor 1; cMG1; early response factor Berg36; EGF-response factor 1; ERF-1; ERF1; mRNA decay activator protein ZFP36L1; Protein TIS11B; RNF162B; TIS11B; TISB; TPA-induced sequence 11b; ZFP36 ring finger protein like 1; ZFP36-like 1; ZFP36L1; zinc finger protein 36, C3H type-like 1; Zinc finger protein 36, C3H1 type-like 1; zinc finger protein, C3H type, 36-like 1 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 45-50 Source/Isotype: Rabbit IgG