CST, 3094S, Aurora B/AIM1 Antibody

Polyclonal Antibody for studying Aurora B. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Aurora B/AIM1 Antibody (CST #3094) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Aurora B/AIM1 Antibody detects endogenous levels of Aurora B/AIM1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human Aurora B/AIM1. Antibodies are purified by protein A and peptide affinity chromatography. Background Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6). Alternate Names AIK2; AIM-1; AIM1; AIRK2; ARK-2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; Aurora 1; Aurora B; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; Aurora-related kinase 2; Aurora/IPL1-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1; STK1; STK12; STK5 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit