CST, 3098T, Phospho-c-Abl (Tyr89) (61A6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Abl1 (Tyr70) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-c-Abl (Tyr89) (61A6) Rabbit Monoclonal Antibody (CST #3098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-c-Abl (Tyr89) (61A6) Rabbit Monoclonal Antibody detects endogenous levels of c-Abl only when phosphorylated at Tyr89. This antibody may cross-react with other tyrosine-phosphorylated proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl. Background The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). Phosphorylation of c-Abl on Tyr89 was identified at Cell Signaling Technology (CST) using PhosphoScan , CST's LC-MS/MS platform for phosphorylation site discovery as well as another publication using MS technology (7). For additional information please visit PhosphoSitePlus , CST's modification site knowledgebase, at www.phosphosite.org. Alternate Names Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; ABL; ABL proto-oncogene 1, non-receptor tyrosine kinase; ABL protooncogene 1 nonreceptor tyrosine kinase; ABL1; BCR-ABL; bcr/abl; bcr/c-abl oncogene protein; c-ABL; c-abl oncogene 1, receptor tyrosine kinase; c-ABL1; CHDSKM; JTK7; p150; Proto-oncogene c-Abl; proto-oncogene tyrosine-protein kinase ABL1; truncated ABL protooncogene 1 nonreceptor tyrosine kinase; Tyrosine-protein kinase ABL1; v-abl; v-abl Abelson murine leukemia viral oncogene homolog 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135 (c-Abl); 210 (Bcr-Abl) Source/Isotype: Rabbit IgG