CST, 30994S, CADM1 Antibody

Polyclonal Antibody for studying CADM1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CADM1 Antibody (CST #30994) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CADM1 Antibody recognizes endogenous levels of total CADM1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CADM1 protein. Antibodies are purified by peptide affinity chromatography. Background CADM1 (cell adhesion molecule 1, TSLC1, Necl-2, IgSF4, SynCAM1) is a member of the immunoglobulin (Ig) cell adhesion superfamily (1). It is a transmembrane protein that is primarily involved in mediating cell-cell adhesion (2,3). CADM1 is comprised of three extracellular Ig-like loops, a transmembrane domain, and a cytoplasmic tail that contains a 4.1 binding motif and a PDZ binding motif. The Ig-like loops contribute to cell-cell junction formation, whereas the cytoplasmic motifs provide structural linkage to the actin cytoskeleton and related adaptor proteins, including actin binding protein 4.1B and membrane-associated guanylate kinases (MAGUKs) (3-5). As an adhesion protein, CADM1 is involved in various biological processes in diverse cells and tissues (6). CADMI functions include structural maintenance and remodeling of epithelium, assembly of neuronal synapses, nerve-mast cell interaction, mast cell behavior, and T cell activation (3,7-9). In many solid tumors, CADM1 has been shown to function as a tumor suppressor by inhibiting malignant proliferation, invasion, and metastasis, while promoting tumor cell apoptosis. In some cases, however, CADM1 has been reported to promote tumor progression (6,10). Alternate Names BL2; CADM1; Cell adhesion molecule 1; DKFZp686F1789; IGSF4; IGSF4A; Immunoglobulin superfamily member 4; immunoglobulin superfamily, member 4; immunoglobulin superfamily, member 4D variant 1; immunoglobulin superfamily, member 4D variant 2; MGC149785; MGC51880; NECL-2; NECL2; nectin-like 2; Nectin-like protein 2; RA175; SgIgSF; Spermatogenic immunoglobulin superfamily; ST17; sTSLC-1; Synaptic cell adhesion molecule; SYNCAM; synCAM1; truncated CADM1 protein; TSLC-1; TSLC1; TSLC1/Nectin-like 2/IGSF4; Tumor suppressor in lung cancer 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit