CST, 31025T, IGFBP1 (D4E9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IGFBP-1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, IGFBP1 (D4E9T) Rabbit Monoclonal Antibody (CST #31025) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 84317 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity IGFBP1 (D4E9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total IGFBP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro240 of human IGFBP1 protein. Background Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. There are six known IGFBP family members (IGFBP1-6), which are structurally related, but encoded by distinct genes. IGFBPs have high affinity for IGFs; in some contexts, IGFBPs inhibit IGF actions by preventing access to IGF receptors, while in others they potentiate IGF actions by facilitating ligand-receptor interaction (1-3). IGFBP1 is produced primarily by the liver and secreted into circulation, and studies show its expression can be negatively regulated by insulin (4, 5). Notably, low levels of IGFBP1 were shown to predict the future onset of Type 2 diabetes (5). Reduced expression of IGFBP1 expression was also associated with tumor progression in breast cancer, prostate cancer, pancreatic cancer and colorectal cancer, possibly stemming from reduced inhibition of mitogenic IGF signaling (6-9). Notably however, other research studies have reported increased levels of IGFBP1 in selected tumor types; in human schwannoma, increased IGFBP1 was associated with stimulation of the integrin β1/FAK pathway, supporting the concept of IGF-independent signaling functions for selected IGFBPs (10,11). Alternate Names AFBP; alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP-1; IBP1; IGF-binding protein 1; IGF-BP25; IGFBP-1; IGFBP1; insulin like growth factor binding protein 1; insulin-like growth factor binding protein 1; Insulin-like growth factor-binding protein 1; Placental protein 12; PP12 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG