CST, 31037SF, LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LDH-A/LDH-C. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31037) is ready to ship. Product Usage Information This product is the carrier free version of product #3558. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3558 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LDHA/LDHC (C28H7) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total LDHA and LDHC proteins. Species cross-reactivity for IHC-P is human only. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. Background Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD . When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD , which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain. While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform (3). Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy (4). Abnormal LDHC expression is associated with several forms of cancer (5). Alternate Names Cancer/testis antigen 32; cell proliferation-inducing gene 19 protein; CT32; epididymis secretory sperm binding protein Li 133P; GSD11; HEL-S-133P; L-lactate dehydrogenase A chain; L-lactate dehydrogenase C chain; lactate dehydrogenase A; lactate dehydrogenase C; lactate dehydrogenase c variant 1; lactate dehydrogenase c variant 3; lactate dehydrogenase c variant 4; lactate dehydrogenase c variant 5; lactate dehydrogenase C4; lactate dehydrogenase M; LDH muscle subunit; LDH testis subunit; LDH-A; LDH-C; LDH-M; LDH-X; LDH1; LDH3; LDHA; LDHC; LDHM; LDHX; MGC111073; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59; testis secretory sperm-binding protein Li 234P Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 37 Source/Isotype: Rabbit IgG