CST, 3103S, SMAD2 (L16D3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Smad2. Validated for Western Blotting,Fluorescent Western,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, SMAD2 (L16D3) Mouse Monoclonal Antibody (CST #3103) is ready to ship. Product Usage Information Western Blotting: 1:1000 Fluorescent Western: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Fluorescent Western Specificity / Sensitivity SMAD2 (L16D3) Mouse mAb detects endogenous levels of total SMAD2 protein and may detect SMAD3 due to high sequence homology. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SMAD2 protein. Background Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8). Alternate Names hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Mouse IgG1