CST, 3108S, Phospho-SMAD2 (Ser465/467) (138D4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Smad2 (Ser465/Ser467) phosphate. Validated for Western Blotting,Simple Western™. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-SMAD2 (Ser465/467) (138D4) Rabbit Monoclonal Antibody (CST #3108) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-SMAD2 (Ser465/467) (138D4) Rabbit Monoclonal Antibody detects endogenous levels of SMAD2 only when dually phosphorylated at serines 465 and 467, and may detect SMAD3 phosphorylated at the equivalent sites. This antibody does not cross-react with other SMAD-related proteins. Species Reactivity: Human, Mouse, Rat, Mink Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser465/467 of human SMAD2. Background Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7 (1-5). Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity (6-8). Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxy-termini (Ser465/467 on Smad2; Ser423/425 on Smad3) by the receptor kinase TGF-β R1 (9-11). Following phosphorylation, Smad2 and Smad3 form a heteromeric complex with the co-Smad family member Smad4. These complexes are translocated to the nucleus where they bind DNA and regulate gene transcription. Alternate Names hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; Mad protein homolog; Mad-related protein 2; MAD, mothers against decapentaplegic homolog 2; MADH2; MADR2; MGC22139; MGC34440; mother against DPP homolog 2; Mothers against decapentaplegic homolog 2; Mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD 2; SMAD family member 2; SMAD, mothers against DPP homolog 2; SMAD2 Specification REACTIVITY: H M R Mi SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG