CST, 3111T, Phospho-VASP (Ser157) Antibody

Polyclonal Antibody for studying VASP (Ser157) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-VASP (Ser157) Antibody (CST #3111) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-VASP (Ser157) Antibody detects endogenous levels of VASP only when phosphorylated at serine 157. This antibody may cross-react with the phosphorylated VASP homologue Mena. Species Reactivity: Human, Mouse, Rat, Monkey, Guinea Pig Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser157 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography. Background Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation, and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site, while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). Alternate Names vasodilator stimulated phosphoprotein; Vasodilator-stimulated phosphoprotein; VASP Specification REACTIVITY: H M R Mk GP SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit