CST, 31135SF, MAGE-A4 (E7O1U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MAGE-A4. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, MAGE-A4 (E7O1U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31135) is ready to ship. Product Usage Information This product is the carrier-free version of product #82491. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 82491 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MAGE-A4 (E7O1U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MAGE-A4 protein. This antibody does not cross-react with MAGE-A2, A3, A6, A10, or A12 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala60 of human MAGE-A4 protein. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). Melanoma-associated antigen-A4 (MAGE-A4) is a cancer testis antigen that belongs to the type I MAGE family of proteins. MAGE-A4 is often overexpressed and mutated in several types of cancer (4-8). Research shows MAGE-A4 promotes aberrant cell growth by preventing p53-dependent cell cycle arrest and apoptosis in normal oral keratinocyte (9). However, MAGE-A4 was also found to interact with the liver oncoprotein gankyrin, suppressing its tumorigenic activity (10). The diverse roles of MAGE-A4 in tumor development may be dependent upon sub-cellular localization (nuclear or cytosolic) and p53 status (6). Due to its upregulated expression in human tumors and high degree of immunogenicity, MAGE-A4 has received significant attention as an immunotherapy target through the use of vaccines and adoptive cell therapy (11-14). Alternate Names Cancer/testis antigen 1.4; cancer/testis antigen family 1, member 4; CT1.4; MAGA4; MAGE family member A4; MAGE-4 antigen; MAGE-41; MAGE-41 antigen; MAGE-A4; MAGE-X2; MAGE-X2 antigen; MAGE4; MAGE4A; MAGE4B; MAGEA4; melanoma antigen family A, 4; melanoma antigen family A4; Melanoma-associated antigen 4; MGC21336 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG