CST, 31235SF, PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying PVR. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31235) is ready to ship. Product Usage Information This product is the carrier free version of product #81254. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 81254 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity PVR/CD155 (D8A5G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD155 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn188 of human CD155 protein. Background Poliovirus receptor (PVR), also known as CD155 and nectin-like 5 (Necl-5), is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR expression may play a role in the invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6). Alternate Names CD155; FLJ25946; HVED; NECL-5; NECL5; nectin-like 5; Nectin-like protein 5; Poliovirus receptor; PVR; PVR cell adhesion molecule; PVS; TAGE4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG