CST, 3124T, Phospho-PDGF Receptor beta (Tyr1009) (42F9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDGFR-b (Tyr1009) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PDGF Receptor beta (Tyr1009) (42F9) Rabbit Monoclonal Antibody (CST #3124) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-PDGF Receptor beta (Tyr1009) (42F9) Rabbit Monoclonal Antibody detects endogenous levels of PDGF receptor β only when phosphorylated at Tyr1009. This antibody may slightly cross-react with other activated PDGF receptor family members and other activated protein tyrosine kinases. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1009 of human PDGF receptor β. Background Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8). Activation of the PDGFRβ leads to autophosphorylation on a number of tyrosine residues, including Tyr1009. Mutation analysis has shown that PDGF-stimulated PLCγ signaling is dependent on autophosphorylation of the PDGFRβ at Tyr1009 and Tyr1021 (9). Phosphorylated Tyr1009 also serves as a binding site for SHP-2, a SH2 domain-containing tyrosine phosphatase that is tyrosine-phosphorylated by PDGFRβ (10). Alternate Names Activated tyrosine kinase PDGFRB; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140B; IBGC4; IMF1; JTK12; KOGS; NDEL1-PDGFRB; PDGF-R-beta; PDGFR; PDGFR beta; PDGFR-1; PDGFR-beta; PDGFR1; PDGFRB; PENTT; PGFRB; platelet derived growth factor receptor beta; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor, beta polypeptide Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit