CST, 31289SF, Notch1 (5B5) Rat Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Notch1. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, Notch1 (5B5) Rat Monoclonal Antibody (BSA and Azide Free) (CST #31289) is ready to ship. Product Usage Information This product is the carrier free version of product #3447. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3447 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Notch1 (5B5) Rat Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Notch1 protein. This antibody recognizes both the full-length (~ 300 kDa) protein and the transmembrane/intracellular region NTM (~120 kDa), which consists of a short extracellular juxtamembrane peptide, a transmembrane sequence and the intracellular domain (NICD). This antibody antibody cannot detect the extracellular (ligand-binding) domain of Notch1 following cleavage at the S2 site by ADAM-type metalloproteases. Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Monoclonal antibody is produced by immunizing animals with a fusion protein corresponding to intracellular residues of Notch1. Background Neuro-oncological ventral antigen (NOVA) 1 and 2 are neuronal RNA-binding proteins (RBPs) that bind YCAY motifs to regulate alternative splicing (1-3). Both proteins have been shown to be targets in paraneoplastic opsoclonus myoclonus ataxia, a neuronal autoimmune disease (1,3). NOVA proteins rely on their KH3 domain to bind RNA and are reciprocally expressed in various parts of the brain (4). NOVA1 has been shown to be essential for neuronal viability, as it controls alternative splicing in GlyRalpha2 and GABA(A) receptor pre-mRNAs (5). NOVA1 is also capable of auto-regulation as it binds to its own exon 4, which contains a phosphorylated protein domain (6). NOVA1/2 double knockout mice are paralyzed due to lack of alternatively spliced agrin Z(+), which helps synapse formation at neuromuscular junctions (7). NOVA2 has been shown to regulate circular RNA biogenesis in the developing brain (8). NOVA1 has been shown to be highly expressed in many different cancer types, and can promote epithelial-mesenchymal transition by binding β-catenin mRNA (9,10). Alternate Names AOS5; AOVD1; hN1; Neurogenic locus notch homolog protein 1; NEXT; NICD; NOTC1; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch homolog 1, translocation-associated; Notch homolog 1, translocation-associated (Drosophila); notch receptor 1; NOTCH1; TAN1; Translocation-associated notch protein TAN-1 Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 120, 300 Source/Isotype: Rat IgG2b