CST, 3128S, ARP2 Antibody

Polyclonal Antibody for studying ARP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ARP2 Antibody (CST #3128) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ARP2 Antibody detects endogenous levels of total ARP2 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, D. melanogaster Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val254 of human ARP2. Antibodies are purified by protein A and peptide affinity chromatography. Background Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division, and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3, and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). Along with N-WASP, the ARP2/3 complex regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3). Alternate Names actin related protein 2; Actin-like protein 2; Actin-related protein 2; ACTR2; ARP2; ARP2 actin related protein 2 homolog; ARP2 actin-related protein 2 homolog (yeast) Specification REACTIVITY: H M R Hm Mk Dm SENSITIVITY: Endogenous MW (kDa): 44 SOURCE: Rabbit