CST, 3130S, c-IAP2 (58C7) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying c-IAP2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, c-IAP2 (58C7) Rabbit Monoclonal Antibody (CST #3130) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity c-IAP2 (58C7) Rabbit Monoclonal Antibody detects endogenous levels of total c-IAP2 protein. No cross reactivity was detected with other family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val117 of human c-IAP2. Background The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2). Alternate Names AIP1; API2; Apoptosis inhibitor 2; baculoviral IAP repeat containing 3; baculoviral IAP repeat-containing 3; Baculoviral IAP repeat-containing protein 3; BIRC3; C-IAP2; Cellular inhibitor of apoptosis 2; CIAP2; HAIP1; hIAP-1; hIAP1; IAP homolog C; IAP-1; IAP1; Inhibitor of apoptosis protein 1; MALT2; mammalian IAP homolog C; MIHC; RING finger protein 49; RING-type E3 ubiquitin transferase BIRC3; RNF49; TNFR2-TRAF signaling complex protein; TNFR2-TRAF-signaling complex protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG