CST, 3145S, Ezrin Antibody

Polyclonal Antibody for studying ezrin. Validated for WB,IP,IHC,IF,F. Highly specific and rigorously validated in-house, Ezrin Antibody (CST #3145) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Ezrin Antibody detects endogenous levels of total ezrin protein. This antibody does not cross-react with ezrin homologues such as radixin and moesin. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human ezrin. Antibodies are purified by protein A and peptide affinity chromatography. Background The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). Alternate Names CVIL; CVL; Cytovillin; cytovillin 2; DKFZp762H157; epididymis secretory protein Li 105; EZR; EZRI; Ezrin; FLJ26216; HEL-S-105; MGC1584; p81; VIL2; villin 2 (ezrin); Villin-2 Specification REACTIVITY: H M R Mk B SENSITIVITY: Endogenous MW (kDa): 81 SOURCE: Rabbit