CST, 31474T, Pellino-1 (D2Z4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PELI1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Pellino-1 (D2Z4F) Rabbit Monoclonal Antibody (CST #31474) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Pellino-1 (D2Z4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total Pellino-1 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala403 of human Pellino-1 protein. Background Pellino-1 (PELI1) is an E3 ubiquitin ligase that regulates activation of various immune pathways in different contexts and cell types (1). Pellino-1 controls NFκB activation downstream of the interleukin-1 receptor and TLRs through association with and ubiquitination of IRAK1 (1-4). Pellino-1 was also demonstrated to regulate the NFκB pathway in T cells by targeting c-Rel for degradation, thereby enabling suppression by regulatory T cells, maintaining peripheral tolerance, and preventing autoimmunity in mice (5). Finally, in experimental autoimmune encephalomyelitis (EAE), the mouse model of multiple sclerosis, Pellino-1 contributes to inflammation by promoting degradation of Traf3 leading to induction of chemokines and proinflammatory cytokines (6). Alternate Names DKFZp686C18116; E3 ubiquitin-protein ligase pellino homolog 1; MGC50990; PELI1; pellino E3 ubiquitin protein ligase 1; pellino homolog 1; pellino homolog 1 (Drosophila); Pellino-1; Pellino-related intracellular-signaling molecule; PRISM; Protein pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG