CST, 31490SF, NaPi2b/SLC34A2 (D6W2G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SLC34A2. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, NaPi2b/SLC34A2 (D6W2G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31490) is ready to ship. Product Usage Information This product is the carrier free version of product #42299. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 42299 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NaPi2b/SLC34A2 (D6W2G) Rabbit Monoclonal Antibody (BSA and Azide Free)recognizes endogenous levels of total NaPi2b/SLC34A2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NaPi2b/SLC34A2 protein. Background The sodium-dependent phosphate transport protein 2B (NaPi-2b, SLC34A2) is a sodium dependent inorganic phosphate (Pi) transporter that regulates phosphate homeostasis in various organs, including the small intestine, lung, liver, and testis (1). In the small intestine, NaPi-2b localizes to the intestinal brush border membrane to mediate Pi reabsorption (2). In the lung, NaPi-2b is expressed in the apical membrane of type II alveolar cells and is involved in the synthesis of surfactant (3). Mutations in the corresponding gene causes pulmonary alveolar microlithiasis, a rare autosomal recessive disorder characterized by the deposition of calcium phosphate microliths throughout the lungs (4). Research studies show aberrant expression of NaPi-2b in various type of cancer, including ovarian, breast, and lung cancer (5). Chromosomal rearrangements involving are seen in gastric carcinoma and non-small cell lung cancer and result in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity (6,7). Alternate Names FLJ90534; Na(+)-dependent phosphate cotransporter 2B; Na(+)/Pi cotransporter 2B; NaPi-2b; NAPI-3B; NAPI-IIb; NaPi3b; NPT2B; NPTIIb; PULAM; SLC34A2; Sodium-dependent phosphate transport protein 2B; Sodium-phosphate transport protein 2B; sodium-phosphate transporter; Sodium/phosphate cotransporter 2B; solute carrier family 34 (sodium phosphate), member 2; solute carrier family 34 (type II sodium/phosphate cotransporter), member 2; Solute carrier family 34 member 2; type II sodium-dependent phosphate transporter 3b Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 73, 90-130 Source/Isotype: Rabbit IgG