CST, 31536L, Protein L (PE-Cy®7 Conjugate)

Miscellaneous for studying in the research area. Product Usage Information Recommended Dilution: 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity Protein L (PE-Cy Species Reactivity: All Species Expected Background Protein L was first isolated from the surface of bacterial species (1). Unlike Protein A and Protein G, which differentially bind to the Fc region of immunoglobulin isotype heavy chains, Protein L contains Ig binding domains that interact with the κ light chain variable domain without disrupting paratope function (2-4). This attribute not only facilitates binding to a broader range of immunoglobulin classes and subclasses than either Protein A or Protein G but also allows for binding to Fab fragments and single chain variable fragments (scFvs) that contain κ light chain sequences. Protein L does, however, display specificity with respect to κ light chain subtypes. In human, Protein L binds to VκI, VκIII, and VκIV subtypes, whereas binding is restricted to VκI in mice (4).