CST, 31544W2, c-Fos (9F6) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)

Monoclonal Antibody for studying Fos. Highly specific and rigorously validated in-house, c-Fos (9F6) Rabbit Monoclonal Antibody (SignalFlex^™ Pacific Blue^™ Conjugate) (CST #31544) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity This antibody detects endogenous levels of total c-Fos protein. The antibody does not cross-react with other Fos proteins, including FosB, FRA1 and FRA2. c-Fos (9F6) Rabbit mAb (SignalFlex™ Pacific Blue™ Conjugate) #2250 non-specifically stains fixed frozen mouse spleen and liver by immunofluorescence. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human c-Fos protein. Background The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3). Alternate Names activator protein 1; AP-1; C-FOS; cellular oncogene c-fos; Cellular oncogene fos; cfos; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; FOS; Fos proto-oncogene, AP-1 trancription factor subunit; Fos proto-oncogene, AP-1 transcription factor subunit; G0/G1 switch regulatory protein 7; G0S7; p55; Protein c-Fos; Proto-oncogene c-Fos; v-fos FBJ murine osteosarcoma viral oncogene homolog Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG