CST, 31647T, Perforin (E3W4I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Perforin mouse. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Perforin (E3W4I) Rabbit Monoclonal Antibody (CST #31647) is ready to ship. Product Usage Information Western Blotting: 1:1000 IHC Leica Bond: 1:100 - 1:400 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 36810 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity Perforin (E3W4I) Rabbit Monoclonal Antibody recognizes endogenous levels of total perforin protein. High expressing cells like CTLL-2 can produce several low molecular weight bands due to degradation of the protein. Non-specific staining was observed in mouse salivary gland by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln63 of mouse perforin protein. Background Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells. Alternate Names Cytolysin; Lymphocyte pore-forming protein; P1; PERF; perforin 1 (pore forming protein); Perforin-1; Pfn; Pfp; pore forming protein; Prf; Prf-1; Prf1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 55-75 Source/Isotype: Rabbit IgG