CST, 31659L, cGAS (D3O8O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying cGAS mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, cGAS (D3O8O) Rabbit Monoclonal Antibody (CST #31659) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity cGAS (D3O8O) Rabbit Monoclonal Antibody recognizes endogenous levels of mouse cGAS protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala66 of mouse cGAS protein. Background Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5). Alternate Names 2'3'-cGAMP synthase; cGAMP synthase; cGAS; Cyclic GMP-AMP synthase; E330016A19Rik; FLJ39829; hypothetical protein LOC214763; m-cGAS; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; Mb21d1; OTTMUSP00000021298; RIKEN cDNA E330016A19 gene Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG