CST, 31754SF, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ZAP70 (Tyr319) phosphate/Syk (Tyr352) phosphate. Validated for WB,IF,F,ELISA+. Highly specific and rigorously validated in-house, Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31754) is ready to ship. Product Usage Information This product is the carrier free version of product #2717. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2717 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of Zap-70 only when phosphorylated at Tyr319. This antibody cross-reacts with endogenous levels of Syk when phosphorylated at Tyr352. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr319 of human Zap-70. Background The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Alternate Names 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; DKFZp313N1010; FLJ17670; FLJ17679; FLJ25043; FLJ37489; IMD48; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SRK; STCD; STD; SYK; Syk-related tyrosine kinase; Tyrosine-protein kinase SYK; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 70, 72 Source/Isotype: Rabbit IgG