CST, 3176S, Progesterone Receptor A/B Antibody

Polyclonal Antibody for studying Progesterone receptor. Validated for Western Blotting. Highly specific and rigorously validated in-house, Progesterone Receptor A/B Antibody (CST #3176) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Progesterone Receptor A/B Antibody detects endogenous levels of total progesterone receptor A and B proteins. This antibody does not cross-react with other PR family members. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr541 of human progesterone receptor. Background Human progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B (1,2). Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms (5). Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation at Ser190 may be critical to its biological function. Alternate Names NR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRGR; Progesterone receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 (PR-A), 118 (PR-B) SOURCE: Rabbit