CST, 3177L, BiP (C50B12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BiP. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 3 sizes. Highly specific and rigorously validated in-house, BiP (C50B12) Rabbit Monoclonal Antibody (CST #3177) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63411 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity BiP (C50B12) Rabbit Monoclonal Antibody detects endogenous levels of total BiP protein. Species Reactivity: Human, Mouse Source / Purification BiP (C50B12) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly584 of human BiP. Background Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4). Alternate Names 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose-regulated protein, 78kDa; GRP-78; GRP78; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; heat shock protein family A (Hsp70) member 5; Heat shock protein family A member 5; Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); HEL-S-89n; HSP70 family protein 5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG