CST, 31793S, PLSCR1 (E9B5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PLSCR1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PLSCR1 (E9B5G) Rabbit Monoclonal Antibody (CST #31793) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PLSCR1 (E9B5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total PLSCR1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PLSCR1 protein. Background Phospholipid scramblase 1 (PLSCR1) is one of four scramblases encoded in the human genome (1). Phospholipid scramblases function to catalyze calcium-mediated ATP-independent mobilization of phospholipids between the inner and outer leaflet of plasma membrane in activated, injured, or apoptotic cells. PLSCR1 is a type II transmembrane protein with a short nine amino acid C-terminal domain and a long N-terminal extracellular domain. Although PLSCR1 is part of a family of proteins that function to catalyze phospholipid mobilization, PLSCR1 may have alternative functions (2,3). Rather, PLSCR1 may function to activate cell signaling pathways, particularly in phagocytic cells of monocyte/macrophage lineage (4). Microglia, resident macrophages of the brain, play a key role in clearance of cellular and pathologic debris, where altered phagocytosis may contribute to neurodegenerative diseases like Alzheimer's disease. PLSCR1 may regulate microglia phagocytosis, representing a potential therapeutic target to modulate microglia phagocytosis (5). Alternate Names Ca(2+)-dependent phospholipid scramblase 1; Erythrocyte phospholipid scramblase; MmTRA1b; Phospholipid scramblase 1; PL scramblase 1; PLS1; PLSCR1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30, 45-100 Source/Isotype: Rabbit IgG