CST, 3183S, BiP Antibody

Polyclonal Antibody for studying BiP. Validated for Western Blotting. Highly specific and rigorously validated in-house, BiP Antibody (CST #3183) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BiP Antibody detects endogenous levels of total BiP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg26 of human BiP protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells (1,2). It was also found to be induced at the protein level by glucose starvation (3). When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding (4). Alternate Names 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose-regulated protein, 78kDa; GRP-78; GRP78; Heat shock 70 kDa protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; heat shock protein family A (Hsp70) member 5; Heat shock protein family A member 5; Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); HEL-S-89n; HSP70 family protein 5; HSPA5; Immunoglobulin heavy chain-binding protein; MIF2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 SOURCE: Rabbit