CST, 31856T, STK25 (E7T2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STK25/YSK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, STK25 (E7T2F) Rabbit Monoclonal Antibody (CST #31856) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STK25 (E7T2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total STK25 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro329 of human STK25 protein. Background STK25 (Serine/threonine protein kinase 25, YSK, SOK1) belongs to the germinal center kinase III (GCKII) subfamily of sterile-20 (STE20) protein kinases (1). STK25 is highly conserved across metazoans, and is reported to be involved in various core cellular processes, including cell migration (2,3) and metabolism (4,5). Along with the closely related MST3 and MST4 kinases, STK25 has been identified as a component of the striatin-interacting phosphatase and kinase (STRIPAK) complex (6). Its role in this regard is one of the mechanisms by which it has been reported to regulate the Hippo signaling pathway (7,8). A defined role for STK25 in lipid metabolism (9) has led some to consider targeting STK25 for the treatment of lipid-related metabolic diseases (10), including non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) (11,12). Alternate Names DKFZp686J1430; serine/threonine kinase 25; serine/threonine kinase 25 (STE20 homolog, yeast); serine/threonine kinase 25 (Ste20, yeast homolog); Serine/threonine-protein kinase 25; SOK-1; SOK1; Ste20-like kinase; Ste20, yeast homolog; Ste20/oxidant stress response kinase 1; sterile 20 (oxidant stress response kinase 1); Sterile 20/oxidant stress-response kinase 1; STK25; yeast Sps1/Ste20-related kinase 1; YSK-1; YSK1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG