CST, 31865T, Ghrelin (D4K1W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ghrelin mouse. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, Ghrelin (D4K1W) Rabbit Monoclonal Antibody (CST #31865) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77865 . Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Ghrelin (D4K1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total ghrelin protein. This antibody detects acylated (n-octanoyl) and non-acylated (desacyl) forms of ghrelin (confirmed by Western blot analysis of synthetic ghrelin protein). Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse ghrelin protein. Background Ghrelin, also known as appetite-regulating hormone, is a neuropeptide hormone belonging to the motilin family. It is the ligand for the growth hormone secretagogue receptor type 1 (GHS-R), expressed by cells in the hypothalamic ventromedial nucleus and arcuate nucleus (1). Ghrelin is synthesized as a preprohormone by ghrelinergic cells in the gastrointestinal tract; proteolytic cleavage yields a 28-amino acid peptide hormone, which undergoes obligate n-octanoylation at serine 3 by the enzyme ghrelin O-acetyltransferase (GOAT) (2). Binding of n-octanoyl ghrelin to GHS-R stimulates growth hormone release, while simultaneously exerting multiple neuroendocrine affects that influence appetite, gastric motility and energy balance (3). Alternate Names 2210006E23Rik; Appetite-regulating hormone; Ghr; Ghrelin; Ghrl; Growth hormone secretagogue; Growth hormone-releasing peptide; m46; Motilin-related peptide; Mtlrp; MTLRPAP; Obestatin; preproghrelin; proghrelin; Protein M46 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG