CST, 31882T, COL4A1 (E9T4L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COL4A1 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, COL4A1 (E9T4L) Rabbit Monoclonal Antibody (CST #31882) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity COL4A1 (E9T4L) Rabbit Monoclonal Antibody recognizes endogenous levels of total COL4A1 protein. This antibody also cross-reacts with bands at 30 kDa and 60 kDa of unknown origin. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to the amino terminus of mouse COL4A1 protein. Background Type IV collagens support extracellular matrix (ECM) function by forming a cross-linking network in the basement membrane zone. There are six different type IV collagens that are encoded by the following genes: , , , , , and . Among them, COL4A1 and COL4A2 are the most common collagens found in the basement membrane, and are usually used as a marker of the basement membrane dynamic stability (1). The type IV collagen fiber exists as a trimeric complex composed of two molecules of COL4A1 and one molecule of COL4A2. The N-terminal 7S domains of the protein function on intermolecular cross-linking, and C-terminal non-collagenous domains (NCI) are responsible for heterotrimer assembly. The trimeric fiber further associates to form a cross-linked sheet structure by NCI/NCI and 7S/7S interaction (2). COL4A1 plays a critical role in maintaining basement membrane integrity for normal tissue functions. Genetic mutations of COL4A1 and COL4A2 have been linked to cardiovascular disease, cerebral small vessel disease, and glomerulocystic kidney disease (3-5). Alternate Names alpha-1 type IV collagen; alpha1(IV) collagen; Arresten; Bru; CO4A1; Col4; Col4a-1; Col4a1; Collagen alpha-1(IV) chain; collagen, type IV, alpha 1; Del(8)44; Del(8)44H; Del(8)Bru44H; OTTMUSP00000023605; procollagen, type IV, alpha 1; Raw; retinal anterior wiring; Svc Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 200, 220 Source/Isotype: Rabbit IgG