CST, 3195L, E-Cadherin (24E10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying E-Cadherin. Validated for WB,WB,IHC,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, E-Cadherin (24E10) Rabbit Monoclonal Antibody (CST #3195) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 IHC Leica Bond: 1:400 - 1:1600 Immunohistochemistry (Paraffin): 1:400 Immunofluorescence (Frozen): 1:1600 Immunofluorescence (Immunocytochemistry): 1:1600 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and Azide Free) version of this product see product # 96743 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity E-Cadherin (24E10) Rabbit Monoclonal Antibody detects endogenous levels of total E-cadherin protein. This antibody does not cross-react with related family members, such as N-cadherin. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Alternate Names Arc-1; BCDS1; CADH1; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); Cadherin E; Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; LCAM; UVO; Uvomorulin Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 135 Source/Isotype: Rabbit IgG