CST, 32015SF, TFE3 (F3X8T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TFE3. Validated for Western Blotting,ELISA+. Highly specific and rigorously validated in-house, TFE3 (F3X8T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #32015) is ready to ship. Product Usage Information This product is the carrier free version of product #19950. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 19950 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TFE3 (F3X8T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TFE3 protein. In addition to the canonical TFE3 protein P19532, this antibody also detects the homologous protein B4DIA5. This antibody detects a band of unknown origin at ~17 kDa. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly511 of human TFE3 protein. Background Transcription factor E3 (TFE3) is a member of a family of basic helix-loop-helix leucine zipper transcription factors that include MITF, TFEB, TFE3, and TFEC. Members of this family form heterodimers with each other, bind the same DNA sequences, and undergo the same types of post-translational modifications, including sumoylation (1). Research studies indicate that TFE3 and other family members play roles in development, organelle biogenesis, nutrient sensing, autophagy, and energy metabolism (2,3). Additional studies report that TFE3 controls the gate for pluripotent cells to exit the state of pluripotency prior to differentiation (4). Translocations involving the gene region have been identified in a number of tumors, including sporadic renal cell tumors. Several specific translocations that result in kidney cancer and involve the gene have been described and characterized in detail (5). Alternate Names BHLHE33; class E basic helix-loop-helix protein 33; DKFZp761J1810; Putative uncharacterized protein DKFZp761J1810; RCCP2; RCCX1; TFE3; TFEA; transcription factor binding to IGHM enhancer 3; transcription factor E family, member A; Transcription factor E3; Transcription factor for IgH enhancer; transcription factor for immunoglobulin heavy-chain enhancer 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 72-89 Source/Isotype: Rabbit IgG