CST, 32042SF, eIF4E (C46H6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying eIF4E. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, eIF4E (C46H6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #32042) is ready to ship. Product Usage Information This product is the carrier free version of product #2067. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For the standard formulation of this product see product # 2067 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity eIF4E (C46H6) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total eIF4E protein. This antibody does not cross-react with 4EHP protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human eIF4E protein. Background Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. Alternate Names AUTS19; CBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; IF4E; MGC111573; mRNA cap-binding protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG