CST, 3216S, Dvl2 Antibody

Polyclonal Antibody for studying DVL2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Dvl2 Antibody (CST #3216) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Dvl2 Antibody detects endogenous levels of total Dvl2 protein. This antibody does not cross-react with Dvl3. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey, Mink Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser638 of human Dvl2. Antibodies are purified by protein A and peptide affinity chromatography. Background Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8). Alternate Names dishevelled 2 (homologous to Drosophila dsh); dishevelled segment polarity protein 2; Dishevelled-2; dishevelled, dsh homolog 2; dishevelled, dsh homolog 2 (Drosophila); DSH homolog 2; DVL2; Segment polarity protein dishevelled homolog DVL-2 Specification REACTIVITY: H M R Hm Mk Mi SENSITIVITY: Endogenous MW (kDa): 90 to 95 SOURCE: Rabbit