CST, 32205T, ARH3 (E8A6G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ADPRHL2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ARH3 (E8A6G) Rabbit Monoclonal Antibody (CST #32205) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ARH3 (E8A6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARH3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro241 of human ARH3 protein. Background ADP-ribosylation is a reversible post-translational modification (PTM) where a single unit or polymers of ADP-ribose (ADPr) groups from β-nicotinamide adenine dinucleotide (NAD ) are transferred onto specific residues of target proteins (1). In addition to proteins, DNA and RNA molecules have also been identified as targets of ADP-ribosylation (2,3). This modification is catalyzed by a diverse group of ADP-ribosyltransferases (ARTs), with the majority of them functioning to add a single ADPr unit (MARylation) while the deposition of multiple ADPrs are done by poly (ADP-ribose) polymerases (PARPs). Reversal of ADP-ribosylation is accomplished by the evolutionarily distinct macrodomain and ADP-ribosylhydrolase (ARH) protein families (4). Specifically, poly (ADP-ribose) glycohydrolase (PARG) or ADP-ribosylhydrolase 3 (ARH3) degrade PARylation down to single ADPr units and MacroD1, MacroD2, TARG1, or ARH1 fully remove them from target residues (5). ADP-ribosylation and the enzymes that regulate it are involved in a wide range of cellular processes, such as DNA repair, chromatin regulation, transcription, and cellular stress response (1,4). Moreover, ADP-ribosylation has been shown to be a therapeutically critical PTM in various cancers, neurodegenerative diseases, and inflammation (6). The ARH family of ADP-ribose hydrolases contains three proteins with similarities in amino acid sequence but differences in their enzymatic activities. ARH3, also known as ADPRHL2, is a ubiquitous protein that localizes to the nucleus, cytosol, and mitochondria in a cell-type specific manner (7-9). ARH3 exhibits partial redundancy with PARG in their preference for hydrolysis of DNA damage-induced serine ADP-ribosylation which underlies a key role for these enzymes in maintaining genomic stability. Arh3-KO mice have been shown to develop increased brain infarction in response to ischemia-reperfusion injury (10) and human ARH3-deficiency is a rare recessive autosomal disorder characterized by neurodegeneration and early death (11,12). Alternate Names [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; ADP-ribose glycohydrolase ARH3; ADP-ribosylarginine hydrolase like 2; ADP-ribosylhydrolase 3; ADP-ribosylhydrolase ARH3; ADP-ribosylhydrolase like 2; ADP-ribosylserine hydrolase; ADPRHL2; ADPRS; ARH3; ARHL2; CONDSIAS; FLJ20446; O-acetyl-ADP-ribose deacetylase ARH3; Poly(ADP-ribose) glycohydrolase ARH3; protein ADP-ribosylarginine hydrolase-like protein 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 39 Source/Isotype: Rabbit IgG