CST, 32253SF, NKX3.1 (D6D2Z) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NKX3.1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, NKX3.1 (D6D2Z) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #32253) is ready to ship. Product Usage Information This product is the carrier free version of product #92998. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 92998 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NKX3.1 (D6D2Z) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NKX3.1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NKX3.1 protein. The epitope is near the amino terminus, in a region that is 100% conserved between isoforms 1 and 5 of human NKX3.1. Background NKX3.1 is a homeobox transcription factor that in mammals plays a defining role in embryonic prostate morphogenesis. The expression of mammalian NKX3.1 is androgen-dependent, restricted primarily to developing and mature prostate epithelium, and is frequently reduced or lost in prostate cancer (1-3). The human gene is located on chromsome 8p21.2, within a region that shows loss of heterozygosity (LOH) in >50% of prostate cancer cases (2). Allelic loss at the locus is also common in high grade Prostate Intraepithelial Neoplasia (PIN), thought to be a putative precursor lesion to invasive prostate adenocarcinomas, suggesting that LOH at the locus is a critical early step in prostate cancer development (4). Notably, the remaining allele is intact in the majority of LOH cases, leading to the suggestion that NKX3.1 functions as a haploinsufficient tumor suppressor (4-6). Due to its highly restricted expression in prostate epithelial cells, NKX3.1 has been suggested as a diagnostic marker of prostate carcinoma (7), and may have additional utility as a biomarker of metastatic lesions originating in the prostate (8). Alternate Names BAPX2; Homeobox protein NK-3 homolog A; Homeobox protein Nkx-3.1; NK homeobox, family 3, A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1; NKX3; NKX3-1; NKX3.1; NKX31; NKX3A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG