CST, 3233T, Phospho-Gab1 (Tyr627) (C32H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Gab1 (Tyr627) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Gab1 (Tyr627) (C32H2) Rabbit Monoclonal Antibody (CST #3233) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Gab1 (Tyr627) (C32H2) Rabbit Monoclonal Antibody detects endogenous levels of Gab1 only when phosphorylated at Tyr627. This antibody may cross-react with phosphorylated Gab2 or Gab3, or with activated receptor tyrosine kinases (RTKs). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr627 of human Gab1. Background The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9). Alternate Names deafness, autosomal recessive 26; DFNB26; GAB1; GRB2 associated binding protein 1; GRB2-associated binder 1; GRB2-associated binding protein 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; symbol withdrawn, see GAB1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG