CST, 32347T, Phospho-FRS2-alpha (Tyr196) (F3O8E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FRS2 (Tyr196) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-FRS2-alpha (Tyr196) (F3O8E) Rabbit Monoclonal Antibody (CST #32347) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-FRS2-alpha (Tyr196) (F3O8E) Rabbit Monoclonal Antibody recognizes endogenous levels of FRS2-α protein only when phosphorylated at Tyr196. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr196 of human FRS2-α protein. Background Fibroblast growth factor receptor substrate 2 (FRS2, also called Suc-associated neurotrophic factor-induced tyrosine-phosphorylated target or SNT) participates in the transmission of extracellular signals from the fibroblast growth factor receptor (FGFR). FGFR activation leads to tyrosine phosphorylation of FRS2 (1). Two FRS2 family members have been identified, FRS2-α (SNT1) and FRS2-β (SNT2) (2), which are phosphorylated by these receptor tyrosine kinases (RTKs). Once phosphorylated, they recruit SH2 domain-containing proteins, including Grb2 and SHP-2 (3,4), mediating downstream signaling. Tyr436 is required for efficient SHP-2 recruitment (5), whereas Tyr196 functions as a docking site for Grb2-Sos complexes (6). Alternate Names epididymis secretory sperm binding protein; FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS1A; FRS2; FRS2A; FRS2alpha; SNT; SNT-1; SNT1; Suc1-associated neurotrophic factor target 1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG