CST, 3251T, Phospho-Caveolin-1 (Tyr14) Antibody

Polyclonal Antibody for studying caveolin-1 (Tyr14) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Caveolin-1 (Tyr14) Antibody (CST #3251) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Caveolin-1 (Tyr14) Antibody detects endogenous levels of caveolin-1 only when phosphorylated at tyrosine 14. This antibody does not cross-react with paxillin, caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr14 of human caveolin-1. Antibodies are purified by protein A and peptide affinity chromatography. Background The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6). Alternate Names BSCL3; CAV; CAV1; caveolin 1; caveolin 1, caveolae protein, 22kDa; Caveolin-1; cell growth-inhibiting protein 32; CGL3; LCCNS; MSTP085; PPH3; VIP21 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 23, 25 SOURCE: Rabbit