CST, 32525S, FIS1 (E3K9O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FIS1. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, FIS1 (E3K9O) Rabbit Monoclonal Antibody (CST #32525) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity FIS1 (E3K9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total FIS1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FIS1 protein. Background Changes in mitochondrial dynamics regulated by environmental cues affect mitochondrial size and shape and have been shown to dramatically impact mitochondrial metabolism, apoptosis, and autophagy (1). Mitochondrial fusion (elongation) and fission (fragmentation) are dynamically controlled by several interacting proteins. Mitochondrial fission is controlled by the GTPase DRP1 which is recruited to the mitochondria by FIS1 and MFF. FIS1 is localized to the mitochondrial outer membrane and overexpression induces fission and leads to apoptosis (2-4). Decreased expression of FIS1 leads to mitochondrial elongation and triggers cellular senescence (5). Elevated expression of FIS1 can also promote autophagy (6). Studies also found that FIS1 interacts with the autophagy initiation protein STX17 and that depletion of FIS1 leads to an increase in mitochondrial localized STX17 and mitophagy (7). Expression of FIS1 has been associated with neurodegeneration and disease, including cancer (8). Alternate Names CGI-135; FIS1; FIS1 homolog; fission 1 (mitochondrial outer membrane) homolog; fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae); fission, mitochondrial 1; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11; TTC11 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG