CST, 3253S, Flotillin-1 Antibody

Polyclonal Antibody for studying flotillin-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Flotillin-1 Antibody (CST #3253) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Flotillin-1 Antibody detects endogenous levels of total flotillin-1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Ile368 of human flotillin-1. Antibodies are purified by peptide affinity chromatography. Background Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located in noncaveolar microdomains (lipid rafts) on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to its colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found in compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, and the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites. However, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. Alternate Names FLOT1; flotillin 1; Flotillin-1; integral membrane component of caveolae Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit