CST, 32563T, Cleaved-PARP (Asp214) (E2T4K) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PARP1 (Asp214, 89 kD) cleaved. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved-PARP (Asp214) (E2T4K) Mouse Monoclonal Antibody (CST #32563) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cleaved-PARP (Asp214) (E2T4K) Mouse Monoclonal Antibody recognizes endogenous levels of the large fragment (89 kDa) of human PARP1 protein produced by caspase cleavage. This antibody does not recognize full-length PARP1 or other PARP isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp214 of human PARP protein. Background PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases (2,3) and is one of the main cleavage targets of caspase-3 (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Alternate Names ADP-ribosyltransferase (NAD+); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; PARP1; poly (ADP-ribose) polymerase; poly (ADP-ribose) polymerase 1; poly (ADP-ribose) polymerase family, member 1; Poly [ADP-ribose] polymerase 1; poly(ADP-ribose) polymerase; poly(ADP-ribose) polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; Poly[ADP-ribose] synthase 1; PPOL; Protein poly-ADP-ribosyltransferase PARP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 89 Source/Isotype: Mouse IgG1