CST, 3256S, CLK3 Antibody

Polyclonal Antibody for studying CLK3. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, CLK3 Antibody (CST #3256) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity CLK3 Antibody detects endogenous levels of full-length and truncated forms of CLK3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr61 of human CLK3 (P49761-1). Antibodies were purified by protein A and peptide affinity chromatography. Background The cdc2-like kinase (CLK) family contains at least four highly conserved isoforms: CLK1, CLK2, CLK3 and CLK4 (1,2). CLKs are dual specificity kinases that autophosphorylate on serine, threonine and tyrosine residues and phosphorylate exogenous substrates on serine and threonine residues (2). CLK family members exist as both a full-length catalytically active form and an alternatively-spliced, inactive truncated form (1). A family of highly phosphorylated proteins, called serine and arginine rich (SR) proteins, are phosphorylated by CLKs (3-5). SR proteins are splicing factors that regulate the assembly of the spliceosome, a macromolecular complex where RNA splicing occurs in the nucleus. They are also involved in the selection of splice sites. Thus, CLKs may play important roles in regulating RNA splicing. CLK3 is abundantly expressed in the testis and, similar to other family members, has been implicated in regulating RNA splicing (6-8). Alternate Names CDC like kinase 3; CDC-like kinase 3; CLK3; Dual specificity protein kinase CLK3; FLJ22858; PHCLK3; PHCLK3/152 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 59, 17 SOURCE: Rabbit