CST, 32600SF, Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ezrin (Thr567) phosphate/radixin (Thr564) phosphate/moesin (Thr558) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #32600) is ready to ship. Product Usage Information This product is the carrier free version of product #3726. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 3726 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (48G2) Rabbit Monoclonal Antibody (BSA and Azide Free) is phospho-specific by peptide-ELISA and exhibits a large signal to noise window. This antibody does not recognize the non-phosphorylated peptide in peptide based ELISA. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr567 of human ezrin protein. Background The ezrin, radixin, and moesin (ERM) proteins function as linkers between the plasma membrane and the actin cytoskeleton and are involved in cell adhesion, membrane ruffling, and microvilli formation (1). ERM proteins undergo intra or intermolecular interaction between their amino- and carboxy-terminal domains, existing as inactive cytosolic monomers or dimers (2). Phosphorylation at a carboxy-terminal threonine residue (Thr567 of ezrin, Thr564 of radixin, Thr558 of moesin) disrupts the amino- and carboxy-terminal association and may play a key role in regulating ERM protein conformation and function (3,4). Phosphorylation at Thr567 of ezrin is required for cytoskeletal rearrangements and oncogene-induced transformation (5). Ezrin is also phosphorylated at tyrosine residues upon growth factor stimulation. Phosphorylation of Tyr353 of ezrin transmits a survival signal during epithelial differentiation (6). Alternate Names CVIL; CVL; Cytovillin; cytovillin 2; DFNB24; DKFZp762H157; epididymis luminal protein 70; epididymis secretory protein Li 105; EZR; EZRI; Ezrin; FLJ26216; HEL-S-105; HEL70; IMD50; Membrane-organizing extension spike protein; MGC1584; MOES; Moesin; MSN; p81; RADI; Radixin; RDX; VIL2; villin 2 (ezrin); Villin-2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 75 Moesin. 80 Ezrin, Radixin. Source/Isotype: Rabbit IgG