CST, 3260S, VRK3 Antibody

Polyclonal Antibody for studying VRK3. Validated for Western Blotting. Highly specific and rigorously validated in-house, VRK3 Antibody (CST #3260) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity VRK3 Antibody detects endogenous levels of total VRK3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human VRK3. Antibodies are purified by protein A and peptide affinity chromatography. Background The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses Erk activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation. Alternate Names Inactive serine/threonine-protein kinase VRK3; Serine/threonine-protein kinase VRK3; Serine/threonine-protein pseudokinase VRK3; vaccinia related kinase 3; Vaccinia-related kinase 3; VRK serine/threonine kinase 3; VRK3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 54 SOURCE: Rabbit